ABS 005-19-02

antibody from Invitrogen Antibodies

Targeting: FCN2 EBP-37, FCNL, ficolin-2, P35

Western blot

ELISA

Immunoprecipitation

Antibody data

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Validation data

Product number: ABS 005-19-02 - Provider product page
Provider: Invitrogen Antibodies
Product name: L-Ficolin Monoclonal Antibody (19)
Antibody type: Monoclonal
Antigen: Purifed from natural sources
Description: ABS 005-19-02 detects L-Ficolin from human samples. This antibody is specific towards L-Ficolin; no cross reactivity is seen with M-Ficolin (Ficolin-1) and H-Ficolin (Ficolin-3). ABS 005-19-02 has been successfully used in ELISA and WB procedures. NOTE: Concentration is lot-dependent and can vary from 0.85-1.15 mg/mL
Reactivity: Human
Host: Mouse
Isotype: IgG
Antibody clone number: 19
Vial size: 400 µL
Concentration: 1 mg/mL
Storage: 4° C, store in dark

FCN2 protein structure - ABS 005-19-02 shown in red.

Supportive validation

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 2 Some older individuals' sera deposit disproportionately little ficolin-2 on serotype 11A bacteria. Sera were evaluated for ficolin-2 deposition on serotype 11A pneumococcus by flow cytometry and for ficolin-2 content by ELISA. NHS, normal human serum collected from a healthy young adult volunteer.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 4 Inhibitors in archived sera interfere with an ELISA to quantify ficolin-2. (A) Ten inhibiting sera and ten noninhibiting sera were selected at random from UAB Study of Aging archived sera and assayed for ficolin-2 by a commercial ELISA. Each data point is shown along with mean and standard deviation. NS, not significant; *, P < 0.05; ***, P < 0.001 by one-way ANOVA with Tukey's post-hoc test. Values for young, healthy controls were previously reported by our laboratory ( Brady et al., 2014a ). (B) Inhibitors in archived sera interfere with a commercial ficolin-2 ELISA. Three normal human sera (NHS1-3) and four inhibiting sera (IS1-4) were assayed by a commercial ELISA alone or in mixture. Mixtures were designed so that observed ficolin-2 would represent the sum of the samples (see Materials and Methods ). Open bars represent the sum of the samples and thus the expected value of the assay. (C) Ficolin-2 immunoblot of sera tested in B suggests that IS1 and IS2 have comparable levels of ficolin-2 to NHS1. Ficolin-2 appears as a band of ~37 kDa under these conditions.